MCNext™ UT DNA Sample Prep Kit
MCNext™ UT DNA Sample Prep Kit

MCNextTM UT DNA Sample Prep Kit provides a fast and easy workflow to prepare up to 96 pooled, indexed paired-end libraries from low input amplicons (>400bp), microbial genomes and plasmids for the subsequent cluster generation and DNA sequencing using  Illumina® NGS sequencing instruments.

 
Name Cat# Description Price Qty
MCNext™ UT DNA Sample Prep Kit
MCUDS-4
4 Samples, 4 Indexes
$150.00 $105.00
MCNext™ UT DNA Sample Prep Kit
MCUDS-24
24 Samples, 10 Indexes
$720.00 $504.00
MCNext™ UT DNA Sample Prep Kit
MCUDS-96
96 Samples, 20 Indexes
$2592.00 $1814.40

Description:

MCNextTM UT DNA Sample Prep Kit provides a fast and easy workflow to prepare up to 96 pooled, indexed paired-end libraries from low input amplicons (>400bp), microbial genomes and plasmids for the subsequent cluster generation and DNA sequencing using  Illumina® NGS sequencing instruments.

The goal of this protocol is to fragmentate and add adapter sequences onto the template DNA with a single tube reaction to generate multiplexed single read or paired-end sequencing libraries.

Features:

Fast Prep Workflow

  • Complete protocol in 80 minutes
  • Enzymatic fragmentation without sonication
  • One-step fragmentation/tagging to save time  

Low Input and High Throughput

  • Only 1 ng input DNA needed
  • Pre-mixed enzymes and nucleotides for multiplexed processing
  • Plate-based processing compatible for up to 96 samples per kit

Higher Coverage Rate and Full Compatibility

  • Directional transposome doubles the sequencing coverage
  • Works on all the Illumina NGS platforms

Best Value on Market

  • Most complete kit with all reagents included
  • All 96 Illumina indices included
  • Standardized library quantification prior to sample pooling and sequencing

Kit Compatibility:
Genome Analyzer IIx
HiScanSQ
NovaSeq 2000
NovaSeq 2500
MiSeq 2000
MiSeq 2500
HiSeq 1000
HiSeq 2000
HiSeq 2500
MiSeq Desktop Sequencer

Kit Components:

4 Sample Kit Contents

Component

Abbreviation

Cap Color

Amount

5 x Tagmentation Buffer

TB

Blue

20 μl

Tagmentation Enzyme

TE

Red

10 μl

Neutralization Buffer

NB

Purple

15 μl

2x Amplification Master Mix

AMM

Clear

110 μl

Resuspension Buffer

RSB

Clear

200 μl

Index 1-1 Primer

N701

Orange

2 μl

Index 1-2 Primer

N702

Orange

2 μl

Index 2-1 Primer

N501

Yellow

2 μl

Index 2-2 Primer

N505

Yellow

2 μl

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

24 Sample Kit Contents

Component

Abbreviation

Cap Color

Amount

5 x Tagmentation Buffer

TB

Blue

110 μl

Tagmentation Enzyme

TE

Red

135 μl

Neutralization Buffer

NB

Purple

85 μl

2x Amplification Master Mix

AMM

Clear

660 μl

Resuspension Buffer

RSB

Clear

1.2ml

Index 1-1 Primer

N701

Orange

4 μl

Index 1-2 Primer

N702

Orange

4 μl

Index 1-3 Primer

N703

Orange

Orange

4 μl

Index 1-4 Primer

N704

4 μl

Index 1-5 Primer

N705

Orange

4 μl

Index 1-6 Primer

N706

Orange

4 μl

Index 2-1 Primer

N501

Yellow

6 μl

Index 2-2 Primer

N502

Yellow

6 μl

Index 2-3 Primer

N503

Yellow

6 μl

Index 2-4 Primer

N504

Yellow

6 μl


96 Sample Kit Contents

Component

Abbreviation

Cap Color

Amount

5 x Tagmentation Buffer

TB

Blue

410 μl

Tagmentation Enzyme

TE

Red

210 μl

Neutralization Buffer

NB

Purple

300 μl

2x Amplification Master Mix

AMM

Clear

1.2 ml x 2

Resuspension Buffer

RSB

Clear

1.2 ml x 4

Index 1-1 Primer

N701

Orange

8 μl

Index 1-2 Primer

N702

Orange

Orange

8 μl

Index 1-3 Primer

N703

8 μl

Index 1-4 Primer

N704

Orange

8 μl

Index 1-5 Primer

N705

Orange

Orange

8 μl

Index 1-6 Primer

N706

8 μl

Index 1-7 Primer

N707

Orange

8 μl

Index 1-8 Primer

N708

Orange

8 μl

Index 1-9 Primer

N709

Orange

8 μl

Index 1-10 Primer

N710

Orange

8 μl

Index 1-11 Primer

N711

Orange

8 μl

Index 1-12 Primer

N712

Orange

8 μl

Index 2-1 Primer

N501

Yellow

12 μl

Index 2-2 Primer

N502

Yellow

12 μl

Index 2-3 Primer

N503

Yellow

12 μl

Index 2-4 Primer

N504

Yellow

12 μl

Index 2-5 Primer

N505

Yellow

12 μl

Index 2-6 Primer

N506

Yellow

12 μl

Index 2-7 Primer

N507

Yellow

12 μl

Index 2-8 Primer

N508

Yellow

12 μl


 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Recommended Storage Condition: -20°C, except Neutralization Buffer @ Room Temperature

Shipping Condition: Dry ice



COA ( Certificate of Analysis )



MSDS & Certificates

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