Antibody biotinylation allows the user to detect protein of interest. This can be useful in localization, western blots, ELISPOT, ELISA and other immunoanalytical methods.
Antibodies come in different varieties known as isotypes or classes. Each one differs in their biological properties, functional locations and ability to deal with different antigens. Knowing the specific isotype of the antibody of interest is crucial for downstream experiments.
ELISA, enzyme-linked immunosorbent assay, is a common biochemistry assay used to detect a substance in a liquid or wet sample. It is a fast and reliable method to evaluate the presence and concentration of an antigen or antibody in a sample.
Most peptides of interest are not adequately large enough to be immunogenic. Conjugation to a carrier protein is usually necessary. KLH, Keyhole Limpet Hemacyanin, is used for conjugation to the peptide of interest because species cross-reactivity is very minimal.
