Description:
These are monospecific antibodies derived from one cell line. They have monovalent affinity towards the same epitope and are generally produced by fusing myeloma cells with the spleen cells from a mouse that has been immunized with the desired antigen.
New Mouse Monoclonal: Balb/c Mice
|
Necessary Purity of ntigen |
*Required Quantity of Antigen |
Products |
Time |
| >80% |
1.5 mg |
Hydridoma cell lines and medium |
4-6 months |
Monoclonal With Hybridoma Cells
| Methods |
Products |
Antibody Concentration |
Time |
| Integra CL350 bioreactor |
36 ml hybridoma medium |
~1mg/ml |
9 weeks |
| Integra CL100 |
90 ml hybridoma medium |
~1mg/ml |
9 weeks |
| Conventional flask culture |
Hybridoma medium |
0.02-0.1 mg/ml |
7-20 days |
| Mouse ascites |
Crude ascites fluid |
20 ml
1-6 mg/ml |
9 weeks |
|