MutS Protein (Thermus aquaticus)
$0.00 – $2,181.03
The MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| MUTST-100 | 100 ug (1 mg/ml) | $311.58 | |||
| MUTST -200 | 1 mg (1 mg/ml) | $2,181.03 | |||
| MUTST -OEM | Any size | Please inquire |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
The MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This Muts protein binds in vitro to heteroduplex DNAs containing mispaired or unpaired bases over a wide temperature range from 4 to 70 °C and has a thermostable ATPase activity. This thermostable Taq MutS is active at temperature between 0 to 75°C. Since Taq MutS efficiently binds to 1-4 bases deletion (or insertion) and mismatch base pairs, it is useful for detecting these mutations. Mutations can be detected in polyacrylamide gels or on a solid phase such as Ni agarose or beads or magnetic Ni-NTA particles.
Applications:
• Remove mismatch DNA (error correction) from gene synthesis reaction
• Mutation detection and removal
• Rapid isothermal SNP detection
Full name:
Thermus aquaticus MutS DNA mismatch repair protein
Source:
expressed in E. coli
Fusion Tag:
6XHis tag at C-terminus
Accession #:
AAC43637
Molecule Weight:
92.8 kDa
Purity:
> 98 % as determined by SDS-PAGE
Storage Buffer:
20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 0.1mM EDTA, 1 mM DTT, 50% Glycerol
Reaction Buffer:
100mM KCl, 50 mM Tris-HCl, pH 8.5, 10 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 2% Glycerol, 65 °C
Storage:
-20°C Avoid repeated freeze-thaw cycles.
References:
1. Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res. 2004 Nov 23;32(20):e162.
2. Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Res. 2005 Mar 30;33(6):e55.
3. MutS as a tool for mutation detection. Acta Biochim Pol. 2005;52(3):575-83. Epub 2005 Aug 4.
4. One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA. Nucleic Acids Res. 2000 Apr 15;28(8):E36.
5. Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology. Nature Methods – 4, 257 – 262 (2007) doi:10.1038/nmeth1007
Additional information
| OPTIONS | 100 ug (1 mg/ml), 1 mg (1 mg/ml), Any size |
|---|
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