FastSep™ NTA Agarose for Phosphoproteins & Phosphopeptides  (C.)
FastSep™ NTA Agarose for Phosphoproteins & Phosphopeptides (C.)

NTA Agarose for Phosphoproteins & Phosphopeptides (Resins)

Also Available FastSep™ High Performance NTA Resins
 
Name Cat# Description Price Qty
Al-NTA Cartridge
ALNTA-CC1X5
5 x 1ml aluminum-charged NTA chromatography cartridge
$99.00
Al-NTA Cartridge
ALNTA-CC5
1 x 5ml aluminum-charged NTA chromatography cartridge
$89.00
Al-NTA Cartridge
ALNTA-CC5X5
5 x 5ml aluminum-charged NTA chromatography cartridge
$369.00
Ga-NTA Cartridge
GANTA-CC1X5
5 x 1ml gallium-charged NTA chromatography cartridge
$99.00
Ga-NTA Cartridge
GANTA-CC5
1 x 5ml gallium-charged NTA chromatography cartridge
$89.00
Ga-NTA Cartridge
GANTA-CC5X5
5 x 5ml gallium-charged NTA chromatography cartridge
$369.00
Fe-NTA Cartridge
FENTA-CC1X5
5 x 1ml iron-charged NTA chromatography cartridge
$99.00
Fe-NTA Cartridge
FENTA-CC5
1 x 5ml iron-charged NTA chromatography cartridge
$89.00
Fe-NTA Cartridge
FENTA-CC5X5
5 x 5ml iron-charged NTA chromatography cartridge
$369.00
Description

FastSep(TM) high performance resins and chromatography cartridges

- Numerous ligand-metal ion combinations 
- High affinity & high capacity 
- Minimal non-specific binding & excellent purity 
- Long spacer arms & minimal steric hindrance 
- Low metal ion leaching & excellent stability 
- Maintain high performance after multiple repeated use 

Application

- For phosphoprotein & phosphopeptide purification 
- Simply replace your existing Al3+, Ga3+, or Fe3+ chelated resin or cartridge, no optimization or protocol changes necessary 
- Purification under native and denaturing conditions 
- Suitable for small proteins, large protein complexes, and proteins with low expression rates 

Purification of Phosphoproteins & Phosphopeptides

Immobilized Al3+, Ga3+, and Fe3+ are the most widely used ions for selective enrichment of phosphopeptides from complex proteolytic digest mixtures containing both phosphorylated and nonphosphorylated components. The most commonly used ligands are NTA and IDA. Depending on identity of proteins, impurity profile and purification conditions, different ligand-metal ion combinations can achieve different purification results. 

How to choose different ligands in IMAC?

NTA and IDA are the two most widely used ligands in IMAC. NTA is a tetravalent ligand with coordination number 4 and therefore stronger coordination of metal ions, while IDA is a trivalent ligand with coordination number 3. Generally, NTA gives higher binding specificity and lower metal ion leaching, while IDA shows more non-specific binding and increased metal ion leaching. IDA can have higher metal ion loading density and usually requires much lower imidazole concentration in eluent. 

As many factors can affect the choice of optimal ligand-metal ion combination, the best approach is to try different combinations and let your proteins decide. FastSep(TM) high performance resins and cartridges offer numerous ligand-metal ion combinations to meet various purification needs. FastSep(TM) high performance resins and chromatography cartridges are based on highly cross-linked agarose 6% beads which have structural characteristics that result in superb physical strength, excellent flow properties, low backpressure, and an open pore structure. FastSep(TM) high performance resins and chromatography cartridges allow operation at very high flow rates, resulting in increased throughput and rapid column cleaning between chromatography runs. 

Specifications

Chromatography technique Phosphoprotein & Phosphopeptide purification
Matrix  Highly cross-linked 6% beaded agarose
Active group Al3+, Ga3+, or Fe3+ charged Nitrilotriacetic Acid (NTA)
Active group density >40 umol/ml drained medium 
Binding capacity >40 mg/ml drained medium
Spacer  17 atom stable and uncharged ether hydrophilic linkage
Bead geometry & size Spherical 50 to 150 um
Bead mean diameter d50v 90 um 
Linear flow velocity <75 cm/h at 25 degC, HR 16/10 column, 5 cm bed height 
Recommended linear flow rate <30 cm/h
Pressure/flow specification Base matrix 100-200 cm/h, pressure drop cm H2O/bed height = 15, bed height 10 cm, 5 cm i.d. 
1ml Cartridge bed dimensions 7×25 mm
1ml Cartridge bed height 25 mm 
1ml Cartridge bed volume 1 ml 
1ml Cartridge column I.D 7 mm
5ml Cartridge bed dimensions 16×25 mm
5ml Cartridge bed height   25 mm
5ml Cartridge bed volume  5 ml 
5ml Cartridge column I.D 16 mm
Maximum cartridge flow rate 5 ml/min (1 ml cartridge) or 20 ml/min (5ml cartridge) 
Recommended cartridge flow rate   1 ml/min (1 ml cartridge) or 5 ml/min (5 ml cartridge)
Maximum pressure during operation 5 bar [0.5 MPa] (70 psi)
pH Stability working range 3 to 13 
pH Stability cleaning in place (cip) 2 to 14
Chemical stability Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane.
Storage 2 to 8 degC

MSDS & Certificates

The Q&A for this product will be available soon.
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