One-step Vector-based pre-miRNA Cloning Systems

One-Step^TM Vector-based Pre-miRNA Cloning Systems

MCLAB provides one-step vector-based pre-miRNA Cloning Systems, which including 2 x PCR master mix, pre-miRNA expression vector and one-step cloning enzyme mix.

For Research Use Only.

Descriptions

The piEXP vector-based pre-miRNA Cloning Systems are convenient systems for the cloning of pre-miRNA products for expression in mammalian cells. The piEXP Vector is prepared by linearized with NheI and BamHI, which greatly improve the efficiency of recombination of a pre-miRNA product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for recombination of pre-miRNA products generated by any thermostable polymerases. A double digestion may be used to release the insert from the vector.

The piEXP Vector-based pre-miRNA System contains linearized pre-miRNA expression vector piEXP/ EmGFP or piEXP/ hLuc, 2 x PCR master mix for PCR amplifying pre-miRNA amplicon, High efficiency (>1 x 10⁹ ) DH5± Competent Cells, one-step^TM cloning/recombination enzyme mix.

• The cloning systems include the PCR 2x master mix, and maker EGFP or Fire fly luciferease
• Clone any pre-miRNA into the vector you choose
• No restriction digestion, phosphatase treatment, or ligation required
• Simple 45 min single-tube reaction on ice protocol
• High Efficiency with >=98% positive clones
• High throughput application

Kit contents

• Box 1: 2 x PCR master mix
• Box 2
  : One-step cloning enzyme mix with piEXP/ EmGFP or piEXP/ hluc
• Box 3
  : High efficiency (>1 x 10⁹) Chemical Competent E. Coli DH5± cells

Storage Temperature:

• Box 1
  : -20°C
• Box 2
  : -20°C
• Box 3
  : -80°C