Choo-Choo PCR Cloning kits

Choo-Choo PCR Cloning Kits®

Choo-Choo PCR Cloning Kits® result	Other PCR Cloning Kits result

Choo-Choo PCR Cloning Kits®Descriptions
Choo-Choo PCR Cloning Kits® is a highly efficient directional PCR cloning kits for rapid, ligase- and restriction enzyme-independent cloning of PCR products. It allows you to clone any PCR fragment into any linearized vector at any location. By a simple incubation on ice, the end of a PCR-generated DNA fragment can precisely fuse to another DNA (vector) end with 6 bp (or more) overlap. The system is so robust: up to 8 PCR-generated DNA fragments can be assembled and cloned into one piece up to 10 kb by one step; The system is highly efficient: 98-100% positive clones.

Choo-Choo PCR Cloning Kits®function
The function of Choo-Choo PCR Cloning Kits® depends on our proprietary enzyme systems. There is no need of restriction enzyme digestion, ligation, and blunt-end polishing. You may limit any extra bases in the final construct. The linearized vector can be generated by PCR or restriction enzyme digestion. The PCR fragments can be generated by Taq DNA polymerase or other high fidelity DNA polymerase. The addition of A by Taq DNA polymerase is not required or has no effect on cloning efficiency. If the PCR product is amplified from plasmid template, it needs gel purified to reduce the background. In addition for PCR cloning, the Choo-Choo PCR Cloning Kits® has other functions, such asfor adaptor, linker and tag addition before and after the inserts, and for gene synthesis.

Choo-Choo PCR Cloning Kits®Features
-Clone any insert, into any location, within any vector you choose
-No restriction digestion, phosphatase treatment, or ligation required
-Multiple fragments up to 8 pieces
-Broad PCR size up to 10 kb
-Final constructs are seamless with no extra or unwanted base pairs
-Simple 45 mins single-tube reaction on ice protocol
-High Efficiency with >=98% positive clones
-Multiple functions: compatible for adaptor, linker and tag addition before or after the inserts; compatible for gene synthesis
-High throughput application

Choo-Choo PCR Cloning Kits®List of Components
Store Box 1 at -80°C.
Store Box 2 at -20°C.
The Choo-Choo Cloning Kits are available in 20 reaction and 100 reaction sizes.

Cat. No. CCK-20 Choo-Choo PCR Cloning Kits (20 reactions)
Box 1:
10x Choo-Choo Cloning Reaction Buffer 40µl
Choo-Choo Cloning Enzyme Mix 40 µl
Box 2:
Choo-Choo Blue chemical Competent cell 20 tubes x 50 µl

Cat. No. CCK-100 Choo-Choo PCR Cloning Kits (100 reactions)
Box 1:
10x Choo-Choo Cloning Reaction Buffer 200 µl
Choo-Choo Cloning Enzyme Mix 200 µl
Box 2:
Choo-Choo Blue chemical Competent cell 100 tubes x 50 µl