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Phi29 DNA Polymerase
Phi29 DNA Polymerase

Phi29 DNA Polymerase is a replicative polymerase. This enzyme has the highest processivity and strand displacement activity among known DNA polymerases - more than 70 kb long DNA stretches can be synthesized.
 
Name Cat# Size Concentration Price Qty
Phi29 DNA Polymerase
PP-100
1,000 units
10,000 U/ml
$109.00
Phi29 DNA Polymerase
PP-200
5,000 units
10,000 U/ml
$327.00
Phi29 DNA Polymerase
PP-OEM
Any Size
please inquire

Description:
Phi29 DNA Polymerase is responsible for the replication of the Bacillus Subtilis phage phi29(1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity(2) and a 3' -> 5' proofreading exonuclease function(3).

Specific Activity: 100,000U/mg

Applications:

  • Catalyzes the removal of 5'-mononucleotides from duplex DNA
  • Replication requiring a high degree of strand displacement and/or processive synthesis
  • High fidelity replication at moderate temperatures

Source:
A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29

Supplied in:
10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.5% Tween-20
0.5% NP-40
50% Glycerol
pH 7.4 @ 25°C

Supplied with:
10x phi29 DNA Polymerase Reaction Buffer

10x phi29 DNA Polymerase Buffer
500 mM Tris-HCl
100 mM (NH4)2SO4
40 mM DTT
100 mM MgCl2
pH 7.5 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C.

Recommended Storage Condition: -20°C

References:
1. Blanco, L. and Salas, M. (1984) Proc. Natl. Acad. Sci. USA, 81, 5325-5329.
2. Blanco, L. et al. (1989) J. Biol. Chem., 264, 8935-8940.
3. Garmendia, C. et al. (1992) J. Biol. Chem., 267, 2594-2599.

Manual & Protocols



COA ( Certificate of Analysis )



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