Phi29 DNA Polymerase
$0.00 – $370.49
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| PP-100 | 2,000 units (10,000 U/ml) | $123.50 | |||
| PP-200 | 10,000 units (10,000 U/ml) | $370.49 | |||
| PP-OEM | Any Size | Please inquire |
- Description
- Additional information
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- Q&A
Description
Description:
Phi29 DNA Polymerase is responsible for the replication of the Bacillus Subtilis phage phi29(1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity(2) and a 3′ -> 5′ proofreading exonuclease function(3).
Specific Activity:
100,000U/mg
Applications:
- Catalyzes the removal of 5′-mononucleotides from duplex DNA
- Replication requiring a high degree of strand displacement and/or processive synthesis
- High fidelity replication at moderate temperatures
Source:
A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29
Supplied in:
10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.5% Tween-20
0.5% NP-40
50% Glycerol
pH 7.4 @ 25°C
Supplied with:
10x phi29 DNA Polymerase Reaction Buffer
10x phi29 DNA Polymerase Buffer
500 mM Tris-HCl
100 mM (NH4)2SO4
40 mM DTT
100 mM MgCl2
pH 7.5 @ 25°C
Unit Definition:
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C.
Recommended Storage Condition:
-20°C
References:
1. Blanco, L. and Salas, M. (1984) Proc. Natl. Acad. Sci. USA, 81, 5325-5329.
2. Blanco, L. et al. (1989) J. Biol. Chem., 264, 8935-8940.
3. Garmendia, C. et al. (1992) J. Biol. Chem., 267, 2594-2599.
Additional information
| OPTIONS | 2,000 units (10,000 U/ml), 10,000 units (10,000 U/ml), Any Size |
|---|
Manual & Protocols
COA ( Certificate of Analysis )
MSDS & Certificates
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