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Poly(A) Polymerase, <i>E. coli.</i>
Poly(A) Polymerase, E. coli.

Poly(A) Polymerase catalyzes the addition of AMP to the 3’ hydroxyl terminus of RNA. Poly(A) Polymerase can add poly(C) and poly(U) to RNA, but does so with much reduced efficiency.
 
Name Cat# Size Concentration Price Qty
Poly (A) Polymerase
PAP-10
1,000 units
5,000U/ml
$125.00
Poly (A) Polymerase
PAP-25
2,000 units
5,000U/ml
$200.00
Poly (A) Polymerase
PAP-50
5,000 units
5,000U/ml
$400.00
Poly (A) Polymerase
PAP-OEM
Any Size
please inquire

Description:
Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3'-hydroxyl of RNA. The reaction requires Mg2+ and is template independent.

Applications:

  • Labeling of RNA with ATP or cordycepin
  • Poly(A) tailing of RNA for cloning or affinity purification
  • Enhances translation of RNA transferred into eukaryotic cells

Source:
The gene encoding E. coli Poly(A) Polymerase expressed in E. coli.

Supplied in:
25 mM Tris-HCl
500 mM NaCl
1 mM MgCl2
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Supplied with:
10x Poly(A) Polymerase Reaction Buffer

10x Poly(A) Polymerase Reaction Buffer:
500 mM Tris-HCl
2.5 M NaCl
100 mM MgCl2
pH 7.9 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.

Recommended Storage Condition: -20°C

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