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Poly(A) Polymerase, E. coli.

Phi29 DNA Polymerase
Placeholder PstI

$0.00 – $453.20

Poly(A) Polymerase catalyzes the addition of AMP to the 3’ hydroxyl terminus of RNA. Poly(A) Polymerase can add poly(C) and poly(U) to RNA, but does so with much reduced efficiency.
SKU: PAP Categories: , , ,
SKU OPTIONS PriceQuantity
PAP-10 1,000 units (5,000U/ml) $141.63
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PAP-25 2,000 units (5,000U/ml) $226.60
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PAP-50 5,000 units (5,000U/ml) $453.20
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PAP-OEM Any Size Please inquire
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Bulk Orders Add selected to cart ✓

Description

Description:
Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3′-hydroxyl of RNA. The reaction requires Mg2+ and is template independent.

Applications:

  • Labeling of RNA with ATP or cordycepin
  • Poly(A) tailing of RNA for cloning or affinity purification
  • Enhances translation of RNA transferred into eukaryotic cells

Source:
The gene encoding E. coli Poly(A) Polymerase expressed in E. coli

Supplied in:
25 mM Tris-HCl
500 mM NaCl
1 mM MgCl2
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Supplied with:
10x Poly(A) Polymerase Reaction Buffer

10x Poly(A) Polymerase Reaction Buffer:
500 mM Tris-HCl
2.5 M NaCl
100 mM MgCl2
pH 7.9 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.

Recommended Storage Condition: -20°C

Additional information

OPTIONS

1,000 units (5,000U/ml), 2,000 units (5,000U/ml), 5,000 units (5,000U/ml), Any Size

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