|
Price: $200 for pack of 25 Traditional Laemmli-based Tris-Glycine Protein Electrophoresis Reproducible Separation of a Broad Range of Proteins Excellent Protein Band ResolutionDescription: SDS-PAGE is a versatile protein electrophoresis application used for the separation of proteins by their size. This allows a relative estimation of the molecular weight of protein samples. MCLAB Tris-Glycine gels are made without SDS, permitting the use of running protein samples under denaturing or nondenaturing conditions. Depending on the application, SDS can be added to the sample and running buffer to create standard SDS-PAGE conditions. Subsequently, the gels can also be used to run proteins on Native-PAGE, without the addition of SDS. This gives the user the ability to perform further analysis of native conformation and activity of protein samples. Gel Selection: Single-percentage gels are best suited for separation of protein samples that are close in molecular weight. When selecting for a single-percentage gel type, keep in mind that your protein samples should be within the lower half of the gel. Gradient gels offer a superior separation when protein samples have a wide range of molecular weights. Thus, high and low molecular weight protein bands can be resolved simultaneously. This is due to the linear decrease in pore size across the entire length of the gel, which allows a sieving effect on a broad range of protein sample sizes. Gradient gels excellently resolves high molecular weight proteins, while restricting excessive separation of low molecular weight proteins. |
