Pst I

Recognition site:
CTGCA^G

Source:
Purified from a strain of E. coli that bears the cloned Pst I gene from Providencia stuartii.

Storage buffer:
10 mM Tris-HCl (pH 7.4), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) Triton X-100.

Reaction conditions:
REACT 2 Buffer at 37°C.

Typical ligation/recut assay results:
Pst I-cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by Pst I.

Recommended storage conditions:
-20°C.

Comments:
Does not cleave DNA when either the A or the 5’ C residue is N6 –methyladenine or 5-methylcytosine, respectively. Partially resistant to heat inactivation (10 min, 65°C). Glycerol concentrations >5% (v/v) may alter specificity.