Puramag™ Plasmid DNA Isolation Kit

The Puramag™ Plasmid DNA Isolation Kit provides rapid isolation of high quality plasmid DNA, BACs, PACs, and cosmids after alkaline-SDS lysis of bacterial cells. The carboxyl coated magnetic beads provide efficient binding of plasmid DNA which is then easily eluted with deionized water or a low salt buffer (Tris-HCl, TE). The system is easily automatable after bacterial lysis and usually results in ~10µg yield of high quality plasmid DNA from 1ml E. Coli cultures. The resulting plasmid DNA after Puramag™ Plasmid is ready for subsequent downstream molecular biology applications including digestion and fluorescent DNA sequencing.

Features

• Quick, simple, and high-throughput (384 samples in <1 hour)
• Easily automated on most robotic platforms
• More cost-effective than column purification
• More robust yield than competitor’s magnetic bead or column based plasmid isolation systems (Fig. 1)
• Results in molecular biology grade plasmid DNA ready for various downstream applications such as restriction digest (Fig 2).

Figure 1: Comparison of Puramag™ beads, Competitor’s beads, and Mini-prep columns in pUC19 and PRNAT plasmid isolation. The plasmids were isolated from pUC19 and PRNAT inoculated 1ml E. coli cultures in LB medium. The cultures were incubated overnight (17 hour) at 37°C.

Figure 2: Gel image showing pUC19 after isolation withPuramag™ beads and subsequent restriction digests. Wells 1and 2 show supercoiled pUC19 plasmid after isolation with Puramag™ Plasmid Isolation kit. Wells 3 and 4 show pUC19 after digest with EcoRI. Wells 5 and 6 show pUC19 after EcoRI and HindIII double digest. The samples were run on a 1% agarose gel in 1X TAE buffer at 120V for 30 minutes.