RNase-Free DNase I

Description:
RNase-free DNase I is an endonuclease that hydrolyzes phosphodiester linkages in DNA to produce mono- and oligodeoxyribonucleotides with 5-phosphate and 3'-OH groups.

Source:
A E. Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I.

Applications:

• Preparation of DNA-free RNA prior to RT-PCR
• Removal of template DNA from RNA in transcription reactions
• DNA labeling by nick-translation when used with DNA Polymerase I
• Studies of DNA-protein interactions by DNase I footprinting

Unit Definition:
One unit is the amount of enzyme required to completely degrade 1 ¼g of plasmid DNA to oligodeoxynucleotides in 10 min at 37°C.

Storage Buffer:
Supplied in 50% glycerol, 10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50% glycerol.

Recommended Storage Conditions:
 -20°C