New special offers are available!
RNase H, <i>E. coli</i>
RNase H, E. coli

RNase H is an endoribonuclease that degrades the RNA strand of RNA/DNA hybrid molecules.
 
Name Cat# Size Concentration Price Qty
RNase H, E. coli
RNHE-100
2,500 units
10,000 U/ml
$134.00
RNase H, E. coli
RNHE-200
10,000 units
10,000 U/ml
$402.00
RNase H, E. coli
RNHE-OEM
Any Size
please inquire

Description:
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5'-phosphate and 3'-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.

Specific Activity: 625,000 U/mg

Applications:

  • Removal of poly(A) tails of mRNA hybridized to poly(dT)
  • Removal of mRNA during second strand cDNA synthesis

Source:
A recombinant E. coli strain carrying the RNAse H gene (rnh) from E. coli


Supplied in:
20 mM Tris-HCl
100 mM KCl
10 mM MgCl2
0.1 mM EDTA
0.1 mM DTT
50% Glycerol
pH 7.9 @ 25°C

Supplied with:
10x RNAse H Buffer

10x RNAse H Buffer:
500 mM Tris-HCl
750 mM KCl
30 mM MgCl2
100 mM DTT
pH 8.3 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.

Recommended Storage Condition: -20°C

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

The Q&A for this product will be available soon.
NGS Library Distribution Kit
Mini Plus Ultrapure Plasmid Prep Kit
Fragment Analysis Matrix
NGS Library Distribution Kit Mini Plus Ultrapure Plasmid Prep Kit Fragment Analysis Matrix
$100.00
$10.00
$100.00
 
RecA Protein, Tth
Q Sepharose High Performance
CHAPSO
RecA Protein, Tth Q Sepharose High Performance CHAPSO
$100.00
$196.20
$188.00