RNase I, E. coli

Description:
RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3’-monophosphates via 2’, 3’ cyclic monophosphate intermediates. Note: The enzyme is inactivated by heating at 70°C for 15 minutes, eliminating phenol extractions to remove the enzyme.

Applications:

• Degradation of single-stranded RNA to mono-, di- and trinucleotide (3)
• Used in ribonuclease protection assays

Source:
An E. coli strain containing a genetic fusion of the RNase I gene (rna) from E. coli and the gene coding for maltose-binding protein (MBP).

Unit Definition:
One unit of enzyme required to catalyze the degradation of 100 ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C.

Storage Buffer:
Supplied in 50% glycerol, 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.01 mM EDTA.

Recommended Storage Conditions:
 -20°C