RNase I, E. coli
$0.00 – $537.04
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| RNIE-100 | 6,000 units (24,000 U/ml) | $179.01 | |||
| RNIE-200 | 24,000 units (24,000 U/ml) | $537.04 | |||
| RNIE-OEM | Any Size | Please inquire |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3′-monophosphates via 2′, 3′ cyclic monophosphate intermediates. Note: The enzyme is inactivated by heating at 70°C for 15 minutes, eliminating phenol extractions to remove the enzyme.
Applications:
- Degradation of single-stranded RNA to mono-, di- and trinucleotide
- Used in ribonuclease protection assays
Source:
An E. coli strain containing a genetic fusion of the RNase I gene (rna) from E. coli and the gene coding for maltose-binding protein (MBP)
Unit Definition:
One unit of enzyme required to catalyze the degradation of 100 ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C.
Storage Buffer:
Supplied in 50% Glycerol, 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.01 mM EDTA.
Recommended Storage Condition: -20°C
Additional information
| OPTIONS | 6,000 units (24,000 U/ml), 24,000 units (24,000 U/ml), Any Size |
|---|
The Q&A for this product will be available soon.
