RNase R
RNase R

RNase R is an E. coli exoribonuclease which exhibits 3' to 5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3 overhangs (less than seven nucleotides).
 
Name Cat# Size Concentration Price Qty
RNase R
RNASR-100
500U
20U/μl
$200.00
RNase R
RNASR-200
2,000U
20U/μl
$650.00
RNase R
RNASR-OEMRNase R
Any Size
please inquire

Description
RNase R is an E. coli exoribonuclease which exhibits 3' to 5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3 overhangs (less than seven nucleotides). RNase R is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing.

Applications
Alternative splicing studies
Gene expression studies
Intron cDNA production
Intronic screening of cDNA libraries
Isolation of splicing intermediates and lariats

Source:
A recombinant E. coli strain carrying the RNAse R gene from E. coli

Storage Buffer:
50 mM Tris-HCl (pH 7.5)
100 mM NaCl
0.1 mM EDTA
0.1% Triton® X-100
1 mM DTT
50% glycerol

10X RNase R Reaction Buffer:
0.2 M Tris-HCl (pH 8.0)
1 M KCl
1 mM MgCl2

Unit Definition: One unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37°C in 20 mM Tris-HCl (pH 8.0), 100 mM KCl and 0.1 mM MgCl2.

Storage Condition:  -20°C

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