RNase R

Description

Description:
RNase R is an E. coli exoribonuclease which exhibits 3′ to 5′ exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven nucleotides). RNase R is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing.

Applications:
• Alternative splicing studies
• Gene expression studies
• Intron cDNA production
• Intronic screening of cDNA libraries
• Isolation of splicing intermediates and lariats

Source:
A recombinant E. coli strain carrying the RNAse R gene from E. coli

Storage Buffer:
50 mM Tris-HCl (pH 7.5)
100 mM NaCl
0.1 mM EDTA
0.1% Triton® X-100
1 mM DTT
50% glycerol

10X RNase R Reaction Buffer:
0.2 M Tris-HCl (pH 8.0)
1 M KCl
1 mM MgCl₂

Unit Definition:
One unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37°C in 20 mM Tris-HCl (pH 8.0), 100 mM KCl and 0.1 mM MgCl₂.

Storage Condition:  -20°C

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