RNase H (rnh) is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.
RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3-monophosphates via 2, 3 cyclic monophosphate intermediates.
Ribonuclease III (Rnase III) is an endoribunuclease that cleaves double-stranded DNA, resulting in 12-15 bp with 2-base, 3-overhangs.
Thermostable RNase H has optimal activity above 65°C and can be used up to 95°C. The enzyme degrades RNA in a DNA:RNA hybrid, maximizing sensitivity and selectivity without affecting DNA or unhybridized RNA.
