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Recombinant  Protein A
Recombinant Protein A

Recombinant Protein A is an immunoglobulin-binding protein derived from the cell wall of the bacterium Staphylococcus aureus. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many of mammalian species, most notably IgGs. Protein A binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b.
 
Name Cat# Description Price Qty
Protein A (Liquid form)
PPA-101
10 mg; 50 mg/ml
$85.00
Protein A (Liquid form)
PPA-102
250 mg; 50 mg/ml
$1800.00
Protein A (Lyophilized)
PPA-201
10 mg
$85.00
Protein A (Lyophilized)
PPA-202
250 mg
$1800.00
Protein A (Lyophilized)
PPA-203
1 g
$3600.00
Protein A (Lyophilized)
PPA-204
10 g
please inquire
Protein A (N-terminal His-tag) (Liquid form)
PPA-103
10 mg; 50 mg/ml
$85.00
Protein A (N-terminal His-tag) (Liquid form)
PPA-104
250 mg; 50 mg/ml
$1800.00
Protein A (N-terminal His-tag) (Lyophilized)
PPA-205
10 mg
$85.00
Protein A (N-terminal His-tag) (Lyophilized)
PPA-206
250 mg
$1800.00
Protein A (N-terminal His-tag) (Lyophilized)
PPA-207
1 g
$3600.00
Protein A (N-terminal His-tag) (Lyophilized)
PPA-208
10 g
please inquire

Description:
Recombinant Protein A is an immunoglobulin-binding protein derived from the cell wall of the bacterium Staphylococcus aureus. It consists of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many of mammalian species, most notably IgGs. Protein A binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. It does not react with human IgG3 or IgD, nor will it react to mouse IgM, IgA or IgE. It binds the heavy chain with the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosisties.

Applications:

  • Antibody purification
  • Protein purification
  • Isolates immune complexes

Source:
Purified from E.coli that carries the protein A gene of Staphylococcus aureus.

Purity:
>98% by SDS-PAGE and HPLC analyses.

Recommended Storage Condition:
-20ºC. Avoid freeze / thaw cycles.

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