Reverse Transcriptase and RNA Polymerases

Reverse Transcriptase and RNA Polymerases

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QuantumScript™ HD Reverse Transcriptase
QuantumScriptTM HD Reverse Transcriptase is a unique mutant version with increased thermostability and reduced RNase H activity. It can be used to synthesize first strand cDNA by reverse transcription (RT) at higher temperatures than other types of Moloney Murine Leukemia Virus (M-MuLV) RT and to reach higher cDNA yields for difficult RNA transcription.
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QuantumScript™ Reverse Transcriptase
Universal Reverse Transcriptase is a mutation with reduced RNase H activity and increased thermostability.
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SmartRT™ Reverse Transcriptase Kit

SmartRTTM Reverse Transcriptase is an engineered MMLV RT that improves the enzyme’s thermostability, reduces RNase H activity and its cDNA synthesis ability. The enzyme also has a terminal transferase activity, where it adds a few extra nucleotides to the end of the synthesized cDNA.  With a 3’ modified oligo dT primer and a 5’  SMART universal oligo containing a terminal complementation to nucleic acids at the 3’  end of the first-strand cDNA, the SmartRTTM reverse transcriptase will produce RACE ready full-length cDNA. 

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First Strand cDNA Synthesis Kit
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA.
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All-In-One 5X Reverse Transcription Mix Kit

MCLAB's All-In-One 5X Reverse Transcription Mix Kit is optimized to synthesize the first strand cDNA for RT-PCR and RT-qPCR.

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T7 High Yield In Vitro Transcription Kit

Phage RNA polymerases are widely used for the in vitro synthesis of RNA transcripts from DNA templates, which have a double-stranded promoter (at least 19 bases) upstream of the sequence to be transcribed.

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RNA Poly(A) Tailing Kit

The RNA Poly(A) Tailing Kit provides a highly pure and active enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3' end of any RNA.

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T7 RNA Polymerase
T7 RNA polymerase is a DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
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 Poly(A) Polymerase, Yeast
Poly(A) Polymerase from Yeast works more efficiently than E. coli Poly(A) Polymerase for RNA oligonucleotide-labeling and poly(A) tailing.
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Poly(A) Polymerase, <i>E. coli.</i>
Poly(A) Polymerase catalyzes the addition of AMP to the 3’ hydroxyl terminus of RNA. Poly(A) Polymerase can add poly(C) and poly(U) to RNA, but does so with much reduced efficiency.
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