Reverse Transcriptase and RNA Polymerases

Reverse Transcriptase and RNA Polymerases

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M-MLV Reverse Transcriptase
M-MuLV Reverse Transcriptase is a DNA polymerase which utilizes RNA as a substrate and exhibits no measurable proofreading 3′→5′ exonuclease function. This enzyme can perform cDNA synthesis by extending off a DNA primer annealed to an RNA template, or can copy a single-stranded DNA template.
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Poly(A) Polymerase, E. coli
Polyn A Polymerase catalyzes the addition of AMP to the 3 hydroxyl terminus of RNA. Poly A Polymerase can add poly(C) and Poly(U) to RNA, but does so with much reduced efficiency.
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T7 RNA Polymerase
Modified enzyme. A DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5'â 3' direction.
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