Description
Ribonuclease III (Rnase III) is an endoribunuclease that cleaves double-stranded DNA, resulting in 12-15 bp with 2-base, 3-overhangs.
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Description: Ribonuclease III (Rnase III) is an endoribonuclease that cleaves double-stranded DNA, resulting in 12-15 bp with 2-base, 3'-overhangs. Source: Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests dsRNA to dsRNA fragments that have two-base, 3' overhangs. Application: - Digestion of dsRNA to short fragments - RNA structure, processing, and maturation studies Unit Definition: One unit of enzyme that will cleave 1 nmol of ribonucleotides in 30 minutes at 37°C. Storage Buffer: Supplied in 50% Glycerol, 50 mM Tris-HCL(pH 7.5),100 mM NaCl, 0.1 mM EDTA. Recommended Storage Conditions: -20°C |
