Rnase III, E. coli

Description:
Ribonuclease III (Rnase III) is an endoribunuclease that cleaves double-stranded DNA, resulting in 12-15 bp with 2-base, 3’-overhangs.

Source:
Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests dsRNA to dsRNA fragments that have two-base, 3´ overhangs.

Applications:

• Digestion of dsRNA to short fragments
• RNA structure, processing, and maturation studies

Unit Definition:
One unit of enzyme that will cleave 1 nmole of ribonucleotides in 30 minutes at 37°C.

Storage Buffer:
Supplied in 50% glycerol, 50mM Tris-HCl (pH 7.5),100mM NaCl, 0.1mM EDTA.

Recommended Storage Conditions:
 -20°C