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MCLAB Redox Running Buffer Agent is used to maintain proteins in a reduced state during electrohphoresis. When performing SDS-PAGE under reducing conditions, Redox Running Buffer Agent should be added to the upper (cathode) buffer chamber. This reagent is optimized for TrenXTM gels, which are set at neutral pH.
4x Sample Buffer is used to prepare protein samples for electrophoresis on TrenXTM gels. A protein sample is mixed with 4x sample buffer (3:1) and heated for 2-5 minutes in boiling water. The SDS denatures the proteins and gives it an overall negative charge. The 2-mercaptoethanol reduces disulfide bonds of the proteins. The bromophenol blue dye serves as a dye front. Preparation using MCLAB Sample Buffer allows protein samples to be separated based on size during electrophoresis.
MCLAB Protein Ladder contains a mixture of 6 highly purified proteins, which are used as a size standard for SDS-PAGE to calculate the molecular weight of a protein of interest. Protein Ladder becomes clearly visible bands from 10-99kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue.
MCLAB premixed solution allows the user to perform fast staining on SDS-PAGE gels for protein analysis. This staining solution is nonhazardous and does not require the use of methanol and acetic acid for destaining. Protein bands become visible within minutes and the whole process takes 30 minutes.
SDS High Molecular Weight Running Buffer is formulated for separating medium- to large-sized protein on TrenXTM gels.
SDS Low Molecular Weight Running Buffer is formulated for separating small- to medium-sized protein on TrenXTM gels.
MCLAB TrenXTM PAGE Gels are precast polyacrylamide gels with a select range of acrylamide percentages.
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