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SP Sepharose Big Beads
Description
SP Sepharose Big Beads are strong ion exchangers designed for industrial applications. The large particle size (100-300 µm) and excellent physical stability of the base matrix ensure maintained speed even with viscous samples. SP Sepharose Big Beads are therefore the ultimate ion exchange media for initial purifications when high viscosity precludes the use of ion exchangers with smaller bead size, such as Sepharose Fast Flow ion exchangers. The unique flow characteristics are also invaluable when adsorption needs to be done quickly.
Feature
- Easy to scale-up
- High flow rates
- High chemical resistance for effective cleaning-in-place (CIP)
- Easy maintenance
| Product Specification |
| Ion exchanger type |
Sulfopropyl strong cation |
| Ionic capacity |
0.18-0.25 mmol (H+)/ml |
| Dynamic capacity |
70 mg RNase/ml medium |
| Pressure/flow spec. |
400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| Average particle size |
90 μm (45-165 μm) |
| Matrix |
Highly cross-linked agarose, 6% |
| pH stability |
3-14 (short term), 4-13 (long term) |
| Chemical stability |
Stable in all common aqueous buffers:
8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH*, and 1 M acetic acid*. |
| Storage |
20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature |
4°C to 30°C |
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