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Standard DNA Sequencing Services

Sample Preparation

Learn about the newly introduced MC Easy Format™ sample submission method for you to save time and money.


For separated DNA templates and primers, detailed requirements are shown below:

Template Type DNA Concentration DNA Volume Primer Concentration Primer Volume
Plasmid 100 ng/l 10 l 3.2 M 10 l
PCR (100-500bp) 5-10 ng/l 10 l 3.2 M 10 l
PCR(500bp-1kb) 10-20 ng/l 10 l 3.2 M 10 l
PCR(>1kb) 20-40 ng/l 10 l 3.2 M 10 l
Cosmid 0.5-1 g/l 20 l 3.2 M 10 l
Fosmid 0.5-1 g/l 20 l 3.2 M 10 l
BAC 0.5-1 g/l 20 l 3.2 M 10 l
Genomic DNA 2-3 g/l 20 l 3.2 M 10 l

For mixed DNA templates and primers, detailed requirements are shown below:

Template Type Amount of DNA Amount of Primer Volume (DNA+Primer)
Plasmid 500 ng 9 pmol 15 l
PCR (100-500bp) 20 ng 9 pmol 15 l
PCR (500bp-1kb) 30 ng 9 pmol 15 l
PCR (>1kb) 50 ng 9 pmol 15 l