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T3 DNA Ligase
T3 DNA Ligase

T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl terminus in duplex DNA. The enzyme joins blunt ends and cohesive ends as well as repair single stranded nicks in duplex DNA.
 
Name Cat# Size Concentration Price Qty
T3 DNA Ligase
T3DL-100
7500 Weiss units
30 Weiss U/ul
$145.00
T3 DNA Ligase
T3DL-200
30,000 Weiss units
30 Weiss U/ul
$435.00
T3 DNA Ligase
T3DL-OEM
Any Size
please inquire

Description:
T3 DNA Ligase joins blunt ends and cohesive ends as well as repairs single-stranded nicks in duplex DNA. In the absence of 20-30% PEG 6000, T3 DNA Ligase displays a very low efficiency for blunt-ended ligation(1). T3 DNA Ligase displays a higher efficiency for joining A/T overhangs than C/G matched ends(1). T3 DNA Ligase retains 95% of its activity in 1.0 M NaCl or KCl, with an optimal concentration of 300 mM(1).

Application:
Catalyzes the formation of a phosphodiester bond between a 5'-phosphate and a 3'-hydroxyl terminus in duplex DNA.

Source:
A recombinant E. coli strain carrying the T3 DNA Ligase gene.

Supplied in:
20 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Supplied with:
2x Rapid Ligation Buffer

2x Rapid Ligation Buffer:
132 mM Tris-HCl
20 mM MgCl2
2 mM DTT
2 mM ATP
15% PEG 8000
pH 7.6
 

Unit Definition:
One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37°C.  Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 0.066 mM ATP, 10 mM DTT, 3.3 µM [32PPi].

One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit.

Specific Activity: 30,000 Weiss Unit/mg

Recommended Storage Condition: -20°C

Reference:
1. Cai, Liang, et al. (2004) J. Biochem. 135, 397-403

Manual & Protocols



COA ( Certificate of Analysis )



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