T4 DNA Helicase

T4 DNA Helicase
Description

T4 replication helicase (gp41) and polymerase (gp43) can be assembled onto a loading site located near the end of a long double-stranded DNA template in the presence of a macro-molecular crowding agent, and that this coupled two-protein system can carry out ATP-dependent strand displacement DNA synthesis at physiological rates (400 to 500 bp per sec) and with high processivity in the absence of other T4 DNA replication proteins.
 
Name Cat# Size Concentration Price Qty
T4 DNA helicase
T4DH-100
10ug
0.5 mg/ml
$265.00
T4 DNA helicase
T4DH-200
25ug
0.5 mg/ml
$375.00
T4 DNA helicase
T4DH-OEM
Any Size
please inquire
Description:
T4 replication helicase (gp41) and polymerase (gp43) can be assembled onto a loading site located near the end of a long double-stranded DNA template in the presence of a macro-molecular crowding agent. This coupled two-protein system can carry out ATP-dependent strand displacement DNA synthesis at physiological rates (400 to 500 bp per sec), with high processivity in the absence of other T4 DNA replication proteins.

Source:
An enzyme from the gene of the bacteriophage T4 phage.

Application:
DNA helicase is an enzyme that aids in DNA synthesis by 'unzipping' the two strands of a DNA helix so that DNA polymerase can access the DNA to add nucleotides and effect copying.

Supplied In:
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

Recommended Storage Conditions: -20°C

Manual & Protocols



COA ( Certificate of Analysis )



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