T4 Endonuclease V

Description:
Also known as T4 PDG, the enzyme has DNA glycosylase and apurinic/apyrimidinic lyase (AP lyase) activity. The protein recognizes cis-syn- cyclobutane pyrimidine dimers caused by UV light. T4 Endonuclease V binds to pyrimidine dimers in double-stranded DNA, then cleaves the glycosyl bond of the 5’-pyrimidine dimer and cleaves the phosphodiester bond 3’ to the resulting basic site.

Applications:

• Used in Single cell gel eletrophoresis
• Studies of DNA damage by UV

Source:
Purified from an E. coli strain carrying a plasmid encoding T4 denV gene.

Unit Definition:
One unit converts 1¼g of UV irradiated supercoiled DNA to nicked plasma in 30 minutes at 37°C. 

Storage Buffer::
Supplied in 50% glycerol, 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.1% Triton X-100 

Recommended Storage Conditions:
 -20°C