T4 Endonuclease VII

T4 Endonuclease VII
Description

Modified Enzyme. Ideal for cleaving DNA heteroduplexes on one strand.
 
Name Cat# Size Concentration Price Qty
T4 Endonuclease VII
TS-100
50 ku
500 U/μl
$235.00
T4 Endonuclease VII
TS-200
100 ku
500 U/μl
$380.00
T4 Endonuclease VII
TS-300
500 ku
500 U/μl
$760.00
T4 Endonuclease VII
TS-OEM
Any Size
please inquire
Description:
T4 Endonuclease VII is a DNA junction specific endonuclease (also referred to as a resolvase or a cleavase). T4 Endonuclease VII functions in vivo to resolve branched DNA structures in newly synthesized DNA. It also recognizes mismatches, insertion or deletion loops, gaps, and apurinic/apyrimidinic sites, and creates nicks in the DNA strands at these sites. The wide variety of substrates accepted suggests that T4 Endonuclease VII recognizes changes in the structure or conformation of DNA rather than binding to a specific sequence. It has also been crystallized as a dimer.

Application:
- Enzymatic mutation detection technology (EMD)
- SNP detection
- Resolve Holliday structures in vitro
- Ideal for cleaving DNA heteroduplexes on one strand

Source:
E. coli strain containing an overproducing clone of the T4 Endonuclease VII protein.

Recommended Reaction Conditions:
50 mM Tris-HCL(pH 7.8 @ 25°C), 10 mM MgCl2, 10 mM DTT 37°C for 30 minutes.

Recommended Storage Conditions:
-20°C. Avoid repeated freeze-thaw.

Reference:
1. Kosak, C., Lee, S., Kemper, B. W. (1998) J Biol Chem, 273(48):31637-31639
2. Kosak H. G., Kemper B. W. (1990) Eur J Biochem. 194(3):779-784

Manual & Protocols



COA ( Certificate of Analysis )



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