T4 Lysozyme

Description:
Bacteriophage T4 Lysozyme breaks down bacterial cell walls. The enzyme attacks the peptidoglycans in the cell walls of bacteria and hydrolyzes the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine. Specific activity of T4 lysozyme is significantly greater than egg white lysozyme when assayed with Micrococcus lysodeikticus and Escherichia coli.

Applications:

• Bacterial lysis for nucleic acid extraction
• Bacterial lysis for recombinant protein extraction

Source:
A recombinant E. coli strain carrying the cloned bacteriophage T4 lysozyme gene.

Unit Definition:
One unit of T4 lysozyme produces a decrease in A350 of 0.008 per second at 23 °C
with a 0.5 mg/ml suspension of lyophilized M. lysodeikticus in 0.02 M phosphate buffer (pH 6.7).

Recommended Storage Conditions:
-20°C

References:

1. Tsugita, A., and Inouye, M. Purification of Bacteriophage T4 Lysozyme. The Journal of Biological Chemistry, 243, 391-397. (1968)