T4 Polynucleotide Kinase
T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules (1).
 
Name Cat# Size Concentration Price Qty
T4 Polynucleotide Kinase
T4PK-100
2,500 units
10,000 U/ml
$134.00
T4 Polynucleotide Kinase
T4PK-200
10,000 units
10,000 U/ml
$402.00
T4 Polynucleotide Kinase
T4PK-OEM;Any Size
please inquire

Description:
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5'-hydroxyl terminus of double- and single-stranded DNA, RNA and nucleoside 3'-monophosphate molecules(1). T4 PNK also exhibits 3'-phosphatase and 2',3'cyclic phosphodiesterase activities.(2-6)

Specific Activity: 133,333 U/mg

Application:
- End-labeling DNA or RNA for probes and DNA sequencing
- Addition of 5'-phosphates to oligonucleotides to allow subsequent ligation
- Removal of 3'-phosphoryl groups

Source:
Purified from a strain of E. coli that expresses the recombinant T4 Polynucleotide Kinase gene.

Supplied in:
10 mM Tris-HCl
50 mM KCl
0.1 µM ATP
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

Supplied with:
10x T4 Polynucleotide Kinase Buffer

10xT4 Polynucleotide Kinase Buffer:
700 mM Tris-HCl
100 mM MgCl2

50 mM DTT
pH 7.6 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [32P] (ATP donor) in 30 minutes at 37°C in 1x T4 Polynucleotide Kinase Reaction Buffer.

Recommended Storage Condition: -20°C

Reference:
1. Richardson, C.C. (1981) P.D. Boyer (Eds.), The Enzymes, 14, pp. 229-314. San Diego: Academic press.
2. Morse, D. P. et al. (1997) Biochemistry 36, 8429-8434.
3. Cameron, V. et al. (1977) Biochemistry 16, 5120-5126.
4. Wand, L. K. et al. (2002) Nucl. Acids Res. 30, 1073-1080.
5. Galburt, E., et al. (2002) Structure 10, 1249-1260.
6. Wang, L. K., et al. (2002) EMBO J. 21, 3873-3880.

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

There is no supporting data for this product. Will be available soon!!
dGTP BrightDyeŽ Terminator Cycle Sequencing Kit
Exonuclease VII
NGS Library Distribution Kit
dGTP BrightDyeŽ Terminator Cycle Sequencing Kit Exonuclease VII NGS Library Distribution Kit
$500.00
$120.00
$100.00
 
Topomize Amplicon Library Prep Kit
McExtract Gel Extraction Kit
BrightDyeŽ Terminator Cycle Sequencing Kit
Topomize Amplicon Library Prep Kit McExtract Gel Extraction Kit BrightDyeŽ Terminator Cycle Sequencing Kit
$280.00
$100.00
$500.00
 
5´ DNA  Adenylation Kit
Cas9 Nuclease
MCMag PCR Purification Kit
5´ DNA Adenylation Kit Cas9 Nuclease MCMag PCR Purification Kit
$80.00
$80.00
$200.00