T4 RNA Ligase 2 (dsRNA Ligase)
T4 RNA Ligase 2 (dsRNA Ligase)

T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA. The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation.
 
Name Cat# Size Concentration Price Qty
T4 RNA Ligase 2 (dsRNA Ligase)
T4RL2-100
2,500 units
10,000 U/ml
$149.00
T4 RNA Ligase 2 (dsRNA Ligase)
T4RL2-200
10,000 units
10,000 U/ml
$447.00
T4 RNA Ligase 2 (dsRNA Ligase)
T4RL2-OEM
Any Size
please inquire

Description:
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), catalyzes both inter- and intramolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 is much more active on joining nicks on double stranded RNA than on joining the ends of single-stranded RNA. The enzyme requires an adjacent 5'-phosphate and 3'-OH for ligation. The enzyme can also ligate the 3'-OH of RNA to the 5'-phosphate of DNA in a double stranded structure.

Source:
An E. coli strain that carries the T4 RNA Ligase 2 gene (I. Schildkraut)

Applications:

  • Ligates a nick in dsRNA
  • Ligates the 3'-OH of RNA to the 5'-phosphate of DNA in a double stranded structure

Reagents Supplied:
T4 Rnl2 Reaction Buffer

Reaction Conditions:
1x T4 Rnl2 Reaction Buffer
Incubate at 37°C

10x T4 RNA Ligase 2 Reaction Buffer:
500 mM Tris-HCl
20 mM MgCl2
10 mM DTT
4 mM ATP
pH 7.5 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37°C.

Unit Assay Conditions:
1x T4 Rnl2 Reaction Buffer and 0.4 µg of an equimolar mix of the 23-mer and 17-mer RNAs. After incubation at 37°C for 30 minutes, the ligated product is detected on a 15% polyacrylamide gel.

Specific Activity: >40,000U/mg
 

Storage Conditions:
10 mM Tris-HCl
50 mM KCl
35 mM (NH4)2SO4
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Recommended Storage Condition: -20°C



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