Description
Modified enzyme. A DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
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Description: T7 RNA Polymerase is a DNA-dependent RNA polymerase derived from the T7 bacteriophage which exhibits a high recognition specificity to the T7 promoter and terminator sequences and catalyzes the 5' -> 3' synthesis of RNA starting at a T7 promoter sequence(1,2). Specific Activity: 312,500 U/mg Application: - Radiolabeled RNA probe preparation - RNA generation for in vitro translation - RNA generation for studies of RNA structure, processing and catalysis - Expression control via anti-sense RNA Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene. Supplied in: 50 mM Tris-HCL 100 mM NaCl 1 mM Dithiothreitol 1 mM EDTA 50% Glycerol 0.1% Triton X-100 pH 7.9 @ 25°C Supplied with: 10x T7 RNA Polymerase Buffer 400 mM Tris-HCL 60 mM MgCl2 100 mM Dithiothreitol 20 mM Spermidine pH 7.9 @ 25°C Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C. Recommended Storage Conditions: -20°C Reference: 1. Chamberlin, M, et al. (1973) J. Biol. Chem. 248, 2235-2244, 2245-2250. 2. Chamberlin, M. et al. (1982) in The Enzymes, 3rd edition, ed. P. D. Boyer (Academic Press, New York.) 15, 87-108. |
