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T7 RNA Polymerase
T7 RNA Polymerase

T7 RNA polymerase is a DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
 
Name Cat# Size Concentration Price Qty
T7 RNA Polymerase
RP-100
25,000 units
100,000 U/ml
$134.00
T7 RNA Polymerase
RP-200
100,000 units
100,000 U/ml
$402.00
T7 RNA Polymerase
RP-OEM
Any Size
please inquire

Description:
T7 RNA Polymerase is a DNA-dependent RNA polymerase derived from the T7 bacteriophage which exhibits a high recognition specificity to the T7 promoter and terminator sequences and catalyzes the 5' -> 3' synthesis of RNA starting at a T7 promoter sequence(1,2).

Specific Activity: 312,500 U/mg

Applications:

  • Radiolabeled RNA probe preparation
  • RNA generation for in vitro translation
  • RNA generation for studies of RNA structure, processing and catalysis
  • Expression control via anti-sense RNA

Source:
Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.

Supplied in:
50 mM Tris-HCl
100 mM NaCl
1 mM DTT
1 mM EDTA
50% Glycerol
0.1% Triton X-100
pH 7.9 @ 25°C

Supplied with:
10x T7 RNA Polymerase Buffer
400 mM Tris-HCl
60 mM MgCl2
100 mM DTT
20 mM Spermidine
pH 7.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.

Recommended Storage Condition: -20°C

References:
1. Chamberlin, M, et al. (1973) J. Biol. Chem. 248, 2235-2244, 2245-2250.
2. Chamberlin, M. et al. (1982) in The Enzymes, 3rd edition, ed. P. D. Boyer (Academic Press, New York.) 15, 87-108.

Manual & Protocols



COA ( Certificate of Analysis )



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