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TAE (Tris/Acetate/EDTA) Buffer, 10X
TAE (Tris/Acetate/EDTA) Buffer, 10X

TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer.

 
Name Cat# Description Price Qty
TAE (Tris/Acetate/EDTA) Buffer, 10X
TAE10-100
1 L
$30.00
TAE (Tris/Acetate/EDTA) Buffer, 10X
TAE10-200
4 L
$82.00
TAE (Tris/Acetate/EDTA) Buffer, 10X
TAE10-OEM
OEM
please inquire

Description:
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer.

Application:
Casting and running buffer for agarose gel electrophoresis of nucleic acids.

Notes:
0.4M Tris base, 0.4M acetate, 10mM EDTA, pH 8.0.

Recommended Storage Condition: Room Temperature

Reference:
1. Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, , (1989) 23, 6.7

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