TEV Protease
TEV Protease

TEV (Tobacco etch virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful reagent for cleaving fusion proteins due to its high specificity and high activity rate.

 
Name Cat# Size Concentration Price Qty
TEV Protease
TEP-100
1 mg
1mg/ml
$180.25
TEV Protease
TEP-200
4 mg
1mg/ml
$540.75
TEV Protease
TEP-OEM
Any Size
please inquire

Description:
TEV (Tobacco Etch Virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful enzyme for cleaving fusion proteins due to its high specificity and its high activity rate. TEV Protease contains a N-terminal His-tag, and it can be removed by Ni-NTA column after cleavage reaction.

Applications:

  • Removal of fusion tags from recombinant proteins
  • Highly dynamic and precise cleavage capabilities
  • purification of proteins and peptides


Supplied With:
TEV substrate, 50 µl
10X TEV buffer

Source:
E. coli derived from Tobacco Etch Virus.

Label: His-tag

Reaction Conditions:
TEV protease is maximally active at 34°C, but its recommended to perform digests at room temperature (20°C) or 4°C. The activity of TEV protease is approximately 3-fold greater at 20°C than at 4°C.

10X TEV buffer
500 mM Tris-HCl, 1.5 M NaCl, pH 8.0

Recommended Storage Condition: -20°C

Figures:
turbotev01.png
Figure 1
turbotev02.png
Figure 2, 69.8 kDa TEV substrate protein is incubated with TurboTEV. The cleaved products are 41.2 kDa and 27.7 kDa, respectively.

Manual & Protocols



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