TEV Protease

Description:
TEV (Tobacco etch virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful reagent for cleaving fusion proteins due to its high specificity and its high activity rate.

Applications:

• Removal of fusion tags from recombinant proteins
• Highly dynamic and precise cleavage capabilities
• purification of proteins and peptides

Source:
E. coli derived from Tobacco etch virus.    

Label:
His

Reaction Conditions:
TEV protease is maximally active at 34 °C, but its recommended to perform digests at room
temperature (20 °C) or 4 °C.  The activity of TEV protease is approximately 3-fold TEV Protease FAQ, 4 of 7 greater at 20 °C than at 4 °C.

Reaction Buffer:
50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT         

Recommended storage conditions:
 -20°C