Thermostable RNase H

Description:
Thermostable RNase H has optimal activity above 65°C and can be used up to 95°C. The enzyme degrades RNA in a DNA:RNA hybrid, maximizing sensitivity and selectivity without affecting DNA or unhybridized RNA.

Applications:

• High hybridization stringency
• Specific hydrolysis of RNA in a DNA:RNA hybrid
• Diagnostic assay of DNA sequences by isothermal probe amplification
• Mapping of mRNA structures

Source:
A recombinant protein purified from E. coli cloned the gene encoding the Thermus thermophilus RNase H.

Unit Definition:
One unit of the enzyme res¼lts in the acid-solubilization of 1 nmole of polyadenylic acid in the presence of an equimolar concentration of polythymidylic acid in 20 minutes at 45°C in 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, and 10 mM MgCl2. Note: The unit assay is performed at 45°C because this is optimal for the Tm of poly(dT):poly(A). The optimal temperature for many applications may be considerably higher.

Storage Buffer :
Supplied in 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton X-100.

Recommended Storage Conditions:
 -20°C