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Topomize Amplicon Library Prep Kit
Topomize Amplicon Library Prep Kit

MCLAB's Topomize Amplicon Library Prep Kit is an innovative, fast and high-quality amplicon library construction kit for next-generation sequencing.

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Description

MCLAB's Topomize Amplicon Library Prep Kit is an innovative, fast and high-quality amplicon library construction kit for next-generation sequencing.   Instead of using traditional ligase-based methods, our kit uses topoisomerase-based technology to attach adapters with barcodes onto the amplicons. The Topomize Amplicon Library Kit has unparalleled efficiency and yield with no adapter dimers or chimeras. The kit is designed for 10 ng to 1 µg of each pooled amplicon DNA input and is compatible with the Illumina platforms. The fast workflow allows the amplicon library to be ready in less than 40 minutes.

Highlights

Easy and cost saving primer design: no linkers or extra nucleotides are needed except the target region primers
More specific amplifications: primers are 100% matched to the target regions
Versatility: adapters with barcode can be attached to any blunt end PCR products
Unbeatable efficiency: Topoisomerase based enzymology generates higher adapter attachment rate with no bias
High quality: no adapter dimer or other chimeras
Fast workflow: get your amplicon library ready in less than 40 min
All-in-One kit: includes all the reagents for sample prep and compatible with Illumina platforms

Topomize Amplicon Library Prep Workflow

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