Description
Detection and identification of single/multiple chromosomal integration sites in transgenic animals.
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Detection and identification of single/multiple chromosomal integration sites in transgenic animals Descriptions
Production of genetically engineered animals has been a successful strategy for generating animal models to better understand the functionality of genes. The site of integration of the transgene within the genome is either a specific or a random event; the integration site will be same or different in each founder animal. Dependents on the delivery system, for some transgene animals, there are typically one insertion site, although multiple transgene copies are often found in a tandem array at that integration site; for other transgene animals, there are often multiple integration events with random transgene insertions on several chromosomes. Determining transgene integration sites is challenging. MCLAB has been developed a proprietary system to isolate DNA fragments adjacent to known sequences. We could determine the precise integration sites, the adjacent sequence (left and right arms) of the transgenic animals from mouse, rat, dog, fish and fruit fly and other animal models.
* A unique way allowing the capture of DNA fragments containing the chromosomal region flanking the transgene. * A quick and precise determination of either single or multiple independent transgene integration sites in founder animals and their offspring. |
