TrenX™ PAGE Gels

TrenX™ PAGE Gels
Description

MCLAB TrenXTM PAGE Gels are precast polyacrylamide gels with a select range of acrylamide percentages.
           
manuals_protocols MSDS        
Manuals & Protocol MSDS        
 
Name Cat# Description Price Qty
TrenX™ PAGE Gels
TPG8-20
30-180kDa (High molecular weight running buffer) or 3.5- 160kDa (Low molecular weight running buffer), 8% Acrylamide, 15 wells, 20ul per well, 10/pk
$50.00
TrenX™ PAGE Gels
TPG10-20
15- 160kDa (High molecular weight running buffer) or 3.5- 160kDa (Low molecular weight running buffer), 10% Acrylamide, 15 wells, 20ul per well, 10/pk
$50.00
TrenX™ PAGE Gels
TPG12-20
3.5- 40kDa (Low molecular weighr running buffer) or 10- 80kDa (High molecular weight running buffer), 12% Acrylamide, 15 wells, 20ul per well, 10/pk
$50.00
TrenX™ PAGE Gels
TPG412-20
15-260kDa (High molecular weight running buffer) or 3.5- 160kDa (Low molecular weight running buffer), 4-12% Acrylamide, 15 wells, 20ul per well, 10/pk
$50.00

Description
MCLAB TrenXTM PAGE Gels are precast polyacrylamide gels with a select range of acrylamide percentages. The precast gels are designed for high performance and provide a wide range of proteins separation. In contrast to traditional Tris-glycine SDS-PAGE gels, TrenXTM PAGE gels are set at a neutral pH environment that result in longer shelf-life and minimize protein modification when run under denaturing conditions. This unique formulation offers reliable separation and excellent resolution of protein bands.

Key Features:
High Resolution: Novel formulation allowing for excellent protein band resolution.
Wide Protein Separation: Low molecular weight or high molecular weight proteins can be separated with the use of high or low molecular weight running buffer.
Long Shelf Life: up to 12 months at 4ºC.
Fast Run Time: 45 minutes or less.

Instructions:

  1. Remove TrenXTM gel from packet and position into the gel running apparatus*.
  2. Pour 200ml of 1X running buffer into the inner gel tank to the rim, and add in 1ml of 200x Redox running buffer agent for reduced samples. Fill sufficient amount of 1X running buffer into the outer gel tank. Total volume should take up ~1000 mL.
  3. Be sure to flush the wells out thoroughly with a transfer pipette or syringe to displace any air bubbles and any storage buffers.
  4. Load prepared protein samples into wells. Optimal sample amount must be established through trials.
  5. Place gel apparatus cover onto gel tank and connect electrodes into power supply. Run gel at constant voltage of 200V for 40 to 50 minutes or until front dye reaches near the bottom of the gel.
  6. Once running the gel is complete, insert a metal spatula into the side of the gel cassette to crack open plastic. Remove gel and proceed to gel staining or transferring.
*Refer to gel systems' manuals for setting up.

Quik-Stain:
MCLAB premixed solution allows the user to perform fast staining on SDS-PAGE gels for protein analysis. This staining solution is nonhazardous and does not require the use of methanol and acetic acid for destaining. Protein bands become visible within minutes and the whole process takes 30 minutes.

Instructions:

  1. Soak gel in a microwavable container with water and microwave for 3 minutes
  2. Discard the hot water and repeat until dye front becomes faded or disappears.
  3. Remove water and add in 50 mL of Coomassie Stain (or enough to completely cover the gel).
  4. Microwave for 1 minute and repeat until protein bands become visible.
  5. Discard the staining solution and replace with water for destatining.
  6. Place a kimwipe on top of gel and microwave for 3 minutes.
  7. Replace with new water and repeat until desired destaining results has been met.

Protein Ladder (10 99 kDa):
MCLAB Protein Ladder contains a mixture of 6 highly purified proteins, which is used as a size standard for SDS-PAGE to calculate the molecular weight of a protein of interest. Protein Ladder becomes clearly visible bands from 10-99 kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue. For general use with 20ul per load.

Cat. No. Name Size
TPS-5 5X Sample buffer 5ml
TPR-ED MES gel running buffer for Denaturing Conditions 1000ml
TPR-OD MOPS gel running buffer for Denaturing Conditions 1000ml
TPL-500 Protein ladder 500ul
TPQ-1L Quik-Stain 1000ml
TPA-15 200x Redox running buffer agent 15ml
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