Description
MCLAB TrenX PAGE Gels are precast polyacrylamide gels with a select range of acrylamide percentages. The precast gels are designed for high performance and provide a wide range of proteins separation. In contrast to traditional Tris-glycine SDS-PAGE gels, TrenX PAGE gels are set at a neutral pH environment that result in longer shelf-life and minimize protein modification when run under denaturing conditions. This unique formulation offers reliable separation and excellent resolution of protein bands.
Key Features:
High Resolution: Novel formulation allowing for excellent protein band resolution.
Wide Protein Separation: Low molecular weight or high molecular weight proteins can be separated with the use of high or low molecular weight running buffer.
Long Shelf Life: up to 12 months at 4ºC.
Fast Run Time: 45 minutes or less.
Instructions:
1 Remove TrenXgel from packet and position into the gel running apparatus*.
2 Pour 200ml of 1X running buffer into the inner gel tank to the rim, and add in 1ml of 200x Redox running buffer agent for reduced samples. Fill sufficient amount of 1X running buffer into the outer gel tank. Total volume should take up ~1000 mL.
3 Be sure to flush the wells out thoroughly with a transfer pipette or syringe to displace any air bubbles and any storage buffers.
4 Load prepared protein samples into wells. Optimal sample amount must be established through trials.
5 Place gel apparatus cover onto gel tank and connect electrodes into power supply. Run gel at constant voltage of 200V for 40 to 50 minutes or until front dye reaches near the bottom of the gel.
6 Once running the gel is complete, insert a metal spatula into the side of the gel cassette to crack open plastic. Remove gel and proceed to gel staining or transferring.
*Refer to gel systems manuals for setting up.
Quik-Stain:
MCLAB premixed solution allows the user to perform fast staining on SDS-PAGE gels for protein analysis. This staining solution is nonhazardous and does not require the use of methanol and acetic acid for destaining. Protein bands become visible within minutes and the whole process takes 30 minutes.
Instructions:
Soak gel in a microwavable container with water and microwave for 3 minutes
Discard the hot water and repeat until dye front becomes faded or disappears.
Remove water and add in 50 mL of Coomassie Stain (or enough to completely cover the gel).
Microwave for 1 minute and repeat until protein bands become visible.
Discard the staining solution and replace with water for destatining.
Place a kimwipe on top of gel and microwave for 3 minutes.
Replace with new water and repeat until desired destaining results has been met.
Protein Ladder (10 – 96 kDa):
MCLAB Protein Ladder contains a mixture of 6 highly purified proteins, which is used as a size standard for SDS-PAGE to calculate the molecular weight of a protein of interest. Protein Ladder becomes clearly visible bands from 10-96 kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue.
For general use with 20ul per load.
