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Turbo HRV 3C Protease
Turbo HRV 3C Protease

MCLAB's HRC3C Protease is fused with both GST and His tags, so that the protease is easily removed form cleavage reactions by either immobilized glutathione (GSH) resin or immobilized Ni-chelating resin. Turbo HRV3C Protease is highly active at 4ºC, and also active in a variety of commonly used buffers, providing more flexibility in experimental design to keep the activity and intact structure of the target proteins.

 
Name Cat# Size Concentration Price Qty
Turbo HRV 3C Protease
THRV3-100
500 units (0.5 mg)
2 mg/ml
$99.00
Turbo HRV 3C Protease
THRV3-200
2,000 units (2 mg)
2 mg/ml
$297.00
Turbo HRV 3C Protease
THRV3-OEM
Any Size
please inquire

Description:
MCLAB's HRC3C Protease is fused with both GST and His tags, so that the protease is easily removed form cleavage reactions by either immobilized glutathione (GSH) resin or immobilized Ni-chelating resin. Turbo HRV3C Protease is highly active at 4ºC, and also active in a variety of commonly used buffers, providing more flexibility in experimental design to keep the activity and intact structure of the target proteins.

Application:
MCLAB's Turbo Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV3C cleavage sequence. HRC3C Protease is highly specific for the PreScission Site, Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro, and cleaves between the Gln and Gly residues.

Supplied With:
HRV 3C Substrate, 100 µl (1 mg/ml)
10x HRV3C Protease Reaction buffer (10 ml)

HRV3C Protease 10X Cleaveage Buffer:
500 mM Tris-HCl (pH 8.0)
1.5 M NaCl

Figure:
Figure 1. SDS-PAGE Analysis of Control Protein by Turbo HRV3C Protease
A 70 kD GST-fusion protein (1) at 1 mg/ml is reacted with Turbo HRV3C Protease at a ratio of 1:100 (w/w) in cleavage buffer at 4ºC for 4 hour (2) 3 days (3) The cleaved products are 45 kD and 25 kD.

Source: E. coli

Supplied In:
50 mM Tris-HCl (pH 8.0)
150 mM NaCl
1 mM EDTA
1 mM DTT
20% glycerol

Unit definition:
1 unit will cleave >95% of 100 µg His-tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.

Recommended Storage Condition: -20°C

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

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