Turbo TEV Protease
$0.00 – $299.11
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| TTP-100 | 0.5 mg (2mg/ml) | $99.70 | |||
| TTP-200 | 2 mg (2mg/ml) | $299.11 | |||
| TTP-OEM | Any Size | Please inquire |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
TurboTEV (Tobacco Etch Virus) Protease is a highly enhanced site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between the Gln and Gly. TurboTEV protease is resistant to auto-inactivation under normal reaction conditions and works as a better catalyst than the wild-type enzyme. It is a very useful enzyme for cleaving fusion proteins due to its high specificity and high activity rate without the requirements of specialized buffer. It has both a His-tag and GST tag, which allow it to be removed by Ni-chelating or GSH resin.
Applications:
- Removal of fusion tags from recombinant proteins
- Highly dynamic and precise cleavage capabilities
- Purification of proteins and peptides
Source:
E. coli derived from Tobacco Etch Virus.
Supplied With:
TEV substrate, 50 µl
10X TEV buffer
Label: His-tag and GST tag
Reaction Conditions:
Turbo TEV protease is maximally active at 34°C, but its recommended to perform digests at room temperature (20°C) or 4°C. The activity of TEV protease is approximately 3-fold greater at 20 °C than at 4°C.
10X TEV buffer
500 mM Tris-HCl, 1.5 M NaCl, pH 8.0
Recommended Storage Condition: -20°C
Figures:
Figure 1
Figure 2. 69.8 kDa TEV substrate protein is incubated with TurboTEV. The cleaved products are 41.2 kDa and 27.7 kDa, respectively.
Additional information
| OPTIONS | 0.5 mg (2mg/ml), 2 mg (2mg/ml), Any Size |
|---|
The Q&A for this product will be available soon.
