TurboTEV Protease

Description:
TurboTEV (Tobacco etch virus) Protease is a highly enhanced site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TurboTEV protease is resistant to auto-inactivation under normal reaction conditions and works as a better catalyst than the wild-type enzyme. It is a very useful reagent for cleaving fusion proteins due to its high specificity and its high activity rate without the requirements of specialized buffer. It has both His tags and GST, which allows it to be removed by Ni-chelating or GSH resin.

Applications:

• Removal of fusion tags from recombinant proteins
• Highly dynamic and precise cleavage capabilities
• purification of proteins and peptides

Source:
E. coli derived from Tobacco etch virus.  

Label:
His and GST

Reaction Conditions:
TEV protease is maximally active at 34 °C, but its recommended to perform digests at room
temperature (20 °C) or 4 °C.  The activity of TEV protease is approximately 3-fold TEV Protease FAQ, 4 of 7 greater at 20 °C than at 4 °C.

Reaction Buffer:
50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT         

Recommended storage conditions:
 -20°C