New special offers are available!
Uracil DNA Glycosylase (UDG)
Uracil DNA Glycosylase (UDG)

Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.

 
Name Cat# Size Concentration Price Qty
Uracil DNA Glycosylase (UDG)
UDG-100
5,000 U
20 U/Ál
$134.00
Uracil DNA Glycosylase (UDG)
UDG-200
20,000 U
20 U/Ál
$402.00
Uracil DNA Glycosylase (UDG)
UDG-OEM
Any Size
please inquire

Description:
Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.

Applications:

  • Control of carry-over contamination in PCR (1)
  • Glycosylase mediated single nucleotide polymorphism detection (GMPD) (2)
  • Site-directed mutagenesis (3)
  • As a probe for protein-DNA interaction studies (4)
  • SNP genotyping
  • Cloning of PCR products (5)
  • Generation of single strand overhangs of PCR products and cDNA

Source:
A recombinant E. coli strain carrying the Uracil DNA Glycosylase gene from E. coli K-12

Specific Activity: 77,000 U/mg

Supplied With:
10X UDG Reaction Buffer
Incubate at 37°C

10X UDG Reaction Buffer:
200mM Tris-HCl
10 mM DTT
10 mM EDTA
pH 8.0 @ 25°C

Supplied In:
10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
50% glycerol
pH 7.5 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of Uracil in 30 minutes from double-stranded, tritiated, Uracil containing-DNA at 37°C in 1X UDG Reaction Buffer.

Recommended Storage Condition: -20°C

References:
1. M. C. Longo et al., Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene. 93, 125-128 (1990).
2. P. Vanghan, T. V. McCarthy, A novel process for mutation detection using uracil DNA glycosylase. Nucleic Acids Res. 26, 810-815 (1998).
3. T. A. Kunkel, Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA. 82, 488-492 (1985).
4. P. R. Devchand, et al., Uracil-DNA glycosylase as a probe for protein-DNA interactions. Nucleic Acids Res. 21, 3437-3443 (1993).
5. P. M. Booth et al., Assembly and cloning of coding sequences for neurotrophic factors directly from genomic DNA using polymerase chain reaction and uracil DNA glycosylase. Gene. 146, 303-308 (1994).

Manual & Protocols



MSDS & Certificates

The Q&A for this product will be available soon.
BL21(DE3) Competent <i>E. coli</i>
JM109 Competent <i>E. coli</i>
BL21(DE3)pLysS Competent <i>E. coli</i>
BL21(DE3) Competent E. coli JM109 Competent E. coli BL21(DE3)pLysS Competent E. coli
$110.00
$110.00
$110.00
 
Lyse-load solution (5x1ml)
Customized Competent <i>E. coli</i>
2X HotSybr qPCR Kit
Lyse-load solution (5x1ml) Customized Competent E. coli 2X HotSybr qPCR Kit
$95.00
$550.00
$100.00
 
2X HotStart PCR Master Mix
T7 RNA Polymerase
CHAPS
2X HotStart PCR Master Mix T7 RNA Polymerase CHAPS
$60.00
$100.00
$88.00