Validated miRNA Expression Constructs

Validated Vector-based miRNA Expression Constructs

Homo sapiens miRNAs (721 clones, Please clik here for the full list)

Descriptions

MicroRNAs (miRNAs) are naturally occurring non-coding RNAs of 21-23 nt and they have been implicated in many biological processes and disease development. MiRNA are processed in multiple steps:

DNA ------------------> Pri-miRNA ---------------------> Pre-miRNA -------------------> Mature miRNA

MCLAB provides clones for over-expression of miRNA of your choice. A region of ~300-500 bp region encompassing each miRNA is cloned downstream of a CMV promoter. Upon transfection, the cellular machinery will process the CMV-driven expression of miRNA precursor into mature miRNA and cellular function can be analyzed.

Features

• Genome-wide miRNA coverage (miRBase release 14.0) - 721 human and 579 mouse
• Express miRNA precursor transcripts in their native sequence context to ensure interaction with endogenous processing machinery leading to authentic mature miRNAs.
• Monitor transduced cells with co-expressed GFP fluorescent marker or firefly Photinus pyralis luciferase . Select cells stably expressing miRNA construct with Neomycin selection marker.
• High quality: fully sequenced expression cassettes
• Convenient: transfection-ready end-toxin free plasmid DNA (10 micrograms)

Kit contents

• 10 micrograms of transfection-ready end-toxin free plasmid DNA in each tube

Storage Temperature:

• -20°C