Validated shRNA plasmid-based Constructs For Research Use Only. Send us the gene list and we will delivered the validated shRNA plasmid-based constructs in 5-7 days. Descriptions A small hairpin RNA or short hairpin RNA ( shRNA ) is a sequence of RNA that makes a tight hairpin turn that can be used to silence gene expression via RNA interference. ShRNA uses a vector introduced into cells and utilizes the U6 promoter to ensure that the shRNA is always expressed. This vector is usually passed on to daughter cells, allowing the gene silencing to be inherited. The shRNA hairpin structure is cleaved by the cellular machinery into siRNA, which is then bound to the RNA-induced silencing complex (RISC). This complex binds to and cleaves mRNAs which match the siRNA that is bound to it.The validated shRNA clone collections of MCLAB are mammalian expression vectorbased small hairpin RNA (shRNA) clones against genome-wide target genes from human, mouse and any other species. MCLAB provides clones for over-expression of shnRNA of your choice. A region encompassing each shRNA is cloned downstream of a U6 promoter. Upon transfection, the cellular machinery will process the expression of shRNA into mature siRNA and cellular function can be analyzed. A set of four expression constructs for every target gene ensures high knockdown efficiency with minimal off-target effects. Features
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