X-Gal Solution
X-Gal Solution

The β-galactosidase substrate X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic stain for β-gal activity, and is commonly used to distinguish between recombinants and nonrecombinants by lacZ α-complementation with appropriate vectors and hosts. X-Gal Solution is provided as a convenient solution with 20 mg/ml concentrate in DMSO, ready for dilution to culture medium or appropriate buffers.

 
Name Cat# Description Price Qty
X-Gal Solution
XGAL-100
10 ml, 20 mg/ml
$60.00
X-Gal Solution
XGAL-OEM
OEM
please inquire

Description:
The β-galactosidase substrate X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic stain for β-gal activity, and is commonly used to distinguish between recombinants and nonrecombinants by lacZ α-complementation with appropriate vectors and hosts. X-Gal Solution is provided as a convenient soution with 20 mg/ml concentrate in DMSO, ready for dilution to culture medium or appropriate buffers.

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

Applications:

  • Blue/white colony screening assay (blue: non-recombinant colonies & white: recombinant colonies).
  • X-Gal staining of eukaryotic cells for the detection of β-galactosidase reporter gene expression.
  • Detection of β-galactosidase activity in various immunological and histochemical applications.

Formula: C14H15BrClNO6

Molecular weight: 408.6

Note:
- Petri dish (9 cm diameter): Spread 40 µL on the surface
- Liquid LB agar: Use 2 µL for each 1 mL of liquid LB agar, add just before pouring plates (at about 50°C)
- Transfected eukaryotic cells: use 25 µL (1 mg) for each 1 mL of staining solution

Recommended Storage Condition: Stable up to 6 months when stored and protected from light at 4°C.

Reference:
1. J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, ed. 3, 2001), pp. 1.124-1.125, A1.27.

MSDS & Certificates

The Q&A for this product will be available soon.
Dh5-Alpha Competent  <em>E. coli </em>
Lyse-load solution (5x1ml)
BL21(DE3) Competent <i>E. coli</i>
Dh5-Alpha Competent E. coli Lyse-load solution (5x1ml) BL21(DE3) Competent E. coli
$110.00
$95.00
$110.00
 
HB101 Competent <i>E. coli</i>
T4 Endonuclease VII
Customized Competent <i>E. coli</i>
HB101 Competent E. coli T4 Endonuclease VII Customized Competent E. coli
$110.00
$200.00
$550.00
 
2X HotStart PCR Master Mix
Exonuclease I (<i>E. coli</i>)
Brij-35 (30% Solution)
2X HotStart PCR Master Mix Exonuclease I (E. coli) Brij-35 (30% Solution)
$60.00
$100.00
$66.00