MCLAB :: Products :: Enzymes :: Serra Nuclease (Benzonase equivalent, 25,000U) HC, Purity

Serra Nuclease HC, Purity > 99%

Catalog No.	Description	Price
BNHC-100	Serra Nuclease (Benzonase equivalent, 25,000U) 250U/ul	$135.00
BNHC-1000	Serra Nuclease (Benzonase equivalent, 250,000U) 250U/ul	$850.00
BNHC-OEM	Serra Nuclease (Benzonase equivalent, Bulk rate) 250U/ul	bulk rate, please inquire

For Research Use Only.

Descriptions

Serra Nuclease (Benzonase equivalent) is a endonuclease from Serratia marcescens. It digests native or heat-denatured DNA and RNA with no proteolytic activity. The ability of Serra Nuclease to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein.

Applications

Viscosity reduction in E. coli lysates
Reduction of sample viscosity for SDS-PAGE gel electrophoresis
Removes nucleic acid contaminants from recombinant protein preparations

Unit Definition
One unit is defined as the amount of enzyme that causes a A260 of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

Storage Buffer
Supplied in 50% glycerol containing 50 mM Tris-HCl pH 8.0, 20 mM NaCl and 2 mM MgCl2

Storage Temperature: -20°C

Reaction Buffer
50% glycerol, 20mM Tris-HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl