2X HotStart PCR Master Mix
Catalog No. |
Size |
Price |
| HMM-050 |
50 Reactions, 10µl/Reaction |
$50.00 | | HMM-100 |
100 Reactions, 10µl/Reaction |
$90.00 |
| HMM-250 |
250 Reactions, 10µl/Reaction |
$188.00 |
| HMM-300 |
600 Reactions, 10µl/Reaction |
$450.00 |
Better than or as good as ABI's and priced 25% less!
For Research Use Only.
DESCRIPTIONS
2X HotStart PCR Master Mix is a premixed 2X concentrated solution of HoTaq DNA Polymerase, reaction buffer, MgCl2 and dNTPs. The DNA template and primers are simply added for PCR reactions. The consistency and efficiency of routine PCR amplifications are optimized.
TECHNOLOGY
The Taq enzyme has been modified to MCLAB's HoTaq in this product. The HoTaq is inactive at room temperature, but become active after 10 minutes at 95ºC.
COMPARISON
Here is the result of comparing MCLAB's HoTaq with some other leading brands.
ADVANTAGES
Better than or as good as ABI's AmpliTaq Gold and at 25% less the cost!
FEATURES
- Hot-start to keep background low
- Solves the primer-dimmer problem
- All the handling can be done at room temperature
- Easy calculation
- Taq DNA Polymerase in ready-to-use mixture
- Low contamination risk
APPLICATIONS
- Regular PCR
- Genotyping
- Multiplex (multiple pairs of primers) PCR
INSTRUCTIONS
Please follow the instructions below for each PCR reaction (20 ul final volume, can be done at room temperature):
- Mix DNA template and primers
- Add H2O to total 10 ul
- Add 10ul 2X HotStart Taq PCR Master Mix
- Set the first PCR step to 95ºC for 10 minutes to active the HoTaq
- Continue PCR as usual
STORAGE
- At -20ºC for long term storage
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