2X HotSybr Real-time PCR KitCatalog No. | Descriptions | Size | Price | Document | HSM400 | Regular level of ROX, for Real-time PCR Machines ABI 7000, 7300, 7700, 7900 | 200 rnx, 4x1.25ml | $255.00 | HSM400 | HSM400LR | Low level of ROX, for Real-time PCR Machines ABI 7500, Stratagene Mx 3000P, Mx 3005P | 200 rnx, 4x1.25ml | $255.00 | HSM400RF | HSM400RF | ROX Free, for Real-time PCR Machines BioRad iCycler MiniOpticon, Opticon 2, Chromo4, iQ5; Roche LightCycler 480; MJ Research DNA Engine Opticon 2, Chromo4; Corbett Roto-gene 3000, 6000 | 200 rnx, 4x1.25ml | $255.00 | HSM400LR | |
For Research Use Only.
PRODUCT
This is a high performance real-time PCR reagent. It utilizes our own proprietary quantitative PCR technology.
ADVANTAGES
1. This product is similar to ABI's Power SYBR® Green PCR Master Mix (CAT# 4367659) but priced 25% less!
2. MCLAB's 2X HotSybr PCR Reaction Mix products cuts 1/2 total reaction time.
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- Normally the total reaction time is 4350 sec: 95°C, 10min => (95°C, 15sec => 60°60sec) x 50
- For MCLAB's 2X HotSybr PCR Reaction Mix, the total reaction time is 2350 sec: 95°C, 10min => (95°C, 5sec => 60°, 30sec) x 50
3. MCLAB's One-step RT-PCR products are faster.
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4. MCLAB's 2X HoTaq PCR Reaction Mix products are superior in amplifying difficult templates.
![]() - This is the amplification of GPIIB gene (70% G+C) .
- 10 ~ 10K copies from 30pg human genomic DNA have been detected.
Applications
Probe based quantitative PCR including DNA quantification, 2-step RT PCR, SNP analysis, etc.
Primer and probe design
Appropriate software, such as ABI Primer ExpressTM, should be used.1. Tm: 58~60°C with 17~25 nucleotides in length
2. To design a small amplicon, <150bp
3. To avoid secondary structures
4. To avoid more than 3 consecutive Gs
5. To avoid complementary 3’ ends
Recommended reaction conditions:
95°C, 10min -> (95°C, 5sec. -> 60°C, 30sec.) for 50 cycles -> melting curve.
Recommended storage condition
-20oC
Notes
To achieve accurate quantification, it is highly recommended to (1) do replicates; (2) reduce pipetting error; (3) primer concentration from 100nM to 300nM; (4) run melting curve following amplification cycles.
Reference
- Higuchi R, Dollinger G, Walsh PS, Griffith R; Bio/Technology 10: 413–417, 1992
FOR RESEARCH USE ONLY.
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