2X HoTaq Real-time PCR KitCatalog No. | Descriptions | Size | Price | Document | HTP400 | Regular level of ROX, for Real-time PCR Machines ABI 7000, 7300, 7700, 7900 | 200 rnx, 4x1.25ml | $266.00 | HTP400 | HTP400LR | Low level of ROX, for Real-time PCR Machines ABI 7500, Mx 3000P, Mx 3005P | 200 rnx, 4x1.25ml | $266.00 | HTP400RF | HTP400RF | ROX Free, for Real-time PCR Machines BioRad iCycler MiniOpticon, Opticon 2, Chromo4, iQ5; Roche LightCycler 480; MJ Research DNA Engine Opticon 2, Chromo4; Corbett Roto-gene 3000, 6000 | 200 rnx, 4x1.25ml | $266.00 | HTP400LR | |
For Research Use Only.
PRODUCT
This is a high performance real-time PCR reagent. It utilizes our own proprietary quantitative PCR technology.
ADVANTAGES
1. MCLAB's this product is similar to ABI's TaqMan® Universal PCR Master Mix, No AmpErase® UNG (CAT# 4324018) but priced 25% less!
2. MCLAB's 2X HotSybr PCR Reaction Mix products cuts down 1/2 total reaction time.
- Normally the total reaction time is 4350 sec: 95°C, 10min => (95°C, 15sec => 60°60sec) x 50
- For MCLAB's 2X HotSybr PCR Reaction Mix, the total reaction time is reduced to 2350 sec: 95°C, 10min => (95°C, 5sec => 60°, 30sec) x 50
3. MCLAB's One-step RT-PCR products are faster.
4. MCLAB's 2X HoTaq PCR Reaction Mix products are superior in amplifying difficult templates.
 - This is the amplification of GPIIB gene (70% G+C) .
- 10 ~ 10K copies from 30pg human genomic DNA have been detected.
Applications
Probe based quantitative PCR including DNA quantification, 2-step RT PCR, SNP analysis, etc.
Primer and probe design
To achieve the best performance, appropriate software, such as ABI Primer ExpressTM, should be used.1.Tm: 60oC for primers and 68oC~70oC for probes with 17~30 nucleotides in length
2.Amplicon size should be small, <150bp
3.To avoid secondary structures in primers and probes
4.To avoid more than 3 consecutive Gs in primers and probes
5.Primers should not have complementary 3’ ends
6.17 ~ 30 nucleotide in length
Recommended reaction conditions:
95oC, 10min. -> (95oC, 5sec. -> 60oC, 30sec.) for 50 cycles.
Recommended storage condition
-20oC
Notes
To achieve accurate quantification, it is highly recommended to do replicates. Also it is important to reduce pipetting error.
Reference
- Holland, P. M., Abramson, R. D., Watson, R., and Gelfand, D. H. 1991. Proceedings of the National Academy of Sciences USA 88:7276–7280.
- Livak, K. J., Flood, S. J. A., Marmaro, J., Giusti, W., and Deetz, K. 1995. PCR Methods and Applications 4:357–362.
- Lee, L. G., Connell, C. R., and Bloch, W. 1993 Nucleic AcidsResearch 21:3761–3766.
FOR RESEARCH USE ONLY.
|
|
Cart is empty
